PCR involves the enzymatic amplification of nucleic acid templates, and can be divided into four major steps, listed below. 1 Denaturation: Double-stranded DNA separates into single strands. Annealing ...

In the polymerase chain reaction (PCR), for example, recognition is provided by sequence-specific hybridization of short oligonucleotides to a larger target, whereas signal amplification requires ...

Rapid PCR-based methods exist that take around 1 hour. CRISPR-Cascade, including sample preparation, requires about half an ...