Primer 3 is complementary to the amplification products bound ... The signal obtained represents the maximal signal possible, as every PCR product on the bead has this sequence at its 5′ end.

PCR involves the enzymatic amplification of nucleic acid templates, and can be divided into four major steps, listed below. 1 Denaturation: Double-stranded DNA separates into single strands. Annealing ...

Conditions of the PCR amplification are as follows ... Even old, degraded DNA yields enough signal to perform the analysis. For maximum signal strength I have optimized the protocol, and several ...

Rapid PCR-based methods exist that take around 1 hour. CRISPR-Cascade, including sample preparation, requires about half an hour, making it the fastest available bloodstream infection diagnostic.

The new CRISPR-Cascade assay enables highly sensitive, multiplexed detection of pathogens within minutes. Read now.