Since the COVID-19 pandemic, pretty much everybody is familiar with this technology: paper-based rapid test strips, also ...

PCR involves the enzymatic amplification of nucleic acid templates, and can be divided into four major steps, listed below. 1 Denaturation: Double-stranded DNA separates into single strands. Annealing ...

Conditions of the PCR amplification are as follows ... Even old, degraded DNA yields enough signal to perform the analysis. For maximum signal strength I have optimized the protocol, and several ...

Primer 3 is complementary to the amplification products bound ... The signal obtained represents the maximal signal possible, as every PCR product on the bead has this sequence at its 5′ end.

Rapid PCR-based methods exist that take around 1 hour. CRISPR-Cascade, including sample preparation, requires about half an ...