3.75 mL of suspension from each bottle was pipetted into a separate 50-mL volumetric flask. About 25 mL of HPLCgrade water was added to each flask, and the flasks were placed on an orbital shaker ...

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After dissolving, the solution was transferred to a 1,000 mL volumetric flask and the volume was adjusted to 1,000 ... The sample was weighed 3 g and placed in a 50 mL centrifuge tube and sealed with ...

Dehydration was performed on the samples using ethanol at 30, 50, 70, and 95% hydroalcoholic solutions ... was added and made up to volume with distilled water in a 10 mL volumetric flask. The ...

After cooling to room temperature, we filtered the hydrolysate, adjusted the volume to 50 mL in a volumetric flask, and mixed thoroughly. We then transferred 1.0 mL of the filtrate to a 15 mL test ...

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