PCR involves the enzymatic amplification of nucleic acid templates, and can be divided into four major steps, listed below. 1 Denaturation: Double-stranded DNA separates into single strands. Annealing ...

In the polymerase chain reaction (PCR), for example, recognition is provided by sequence-specific hybridization of short oligonucleotides to a larger target, whereas signal amplification requires ...

Conditions of the PCR amplification are as follows ... Even old, degraded DNA yields enough signal to perform the analysis. For maximum signal strength I have optimized the protocol, and several ...

Rapid PCR-based methods exist that take around 1 hour. CRISPR-Cascade, including sample preparation, requires about half an ...