Centrifuge for 10 min, and pour the supernatant into a 50-mL volumetric flask. Repeat this step twice and then make up to the mark. Adjust the optical density (OD) measurement at a wavelength of 625 ...

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After dissolving, the solution was transferred to a 1,000 mL volumetric flask and the volume was adjusted to 1,000 ... The sample was weighed 3 g and placed in a 50 mL centrifuge tube and sealed with ...