In this study, we show a facile elution strategy for Protein A chromatography based on amino acid-induced pH shift to control the pH of the final elution pool effectively, protecting the eluted ...

IT is well known that the application of long-chain fatty acid samples to gas chromatography needs their ... The esterification of the fatty acids with diazomethane, or methanol–hydrochloric ...

Since this disorder may not always be detectable by a simple ninhydrin screening test on urine 18 diagnosis may well depend on 2-dimensional amino acid chromatography. Our experience once again ...

An aliquot of the ketoacid extract was also hydrogenated in an acid medium in an electrolytic desalter; partition chromatography was then used to identify the regenerated amino acids. Note the ...

The ninhydrin reaction, generally prescribed by pharmacopoeias for the TLC detection of amino acids, is worth illustrating although TLC analyses of amino acids are not part of the laboratory ...

Amino acid analyses performed after salt removal revealed a preponderance of acidic amino acids and 35 to 38 unknown ninhydrin reactive peaks. Following acid hydrolysis, the unknown peaks decreased in ...

and then the free amino acid contents were measured by an amino acid analyzer (L-8900, Hitachi Ltd., Tokyo, Japan) by the post-column derivatization of ninhydrin. Amino acids were expressed as mg/100 ...